Multidrug-resistant Tumor Cells by Monoclonal Antibodies Resistance-associated Protein MRP in Human Immunochemical Detection of the Multidrug
نویسندگان
چکیده
We have generated rat and murine monoclonal antibodies against multidrug resistance-associated protein (MRP), a M,. 180,000-195,000 membrane glycoprotein involved in a non-P-glycoprotein multidrug re sistance of human tumor cells. The antibodies were raised against two different segments of MRP and found to be suitable for protein blot analyses, immunohistochemical and cytochemical studies, as well as flow cytometry of permeabilized cells. The antibodies do not cross-react with the human P-glycoproteins. Immunocytochemistry using l/AT-<>viTf\pressing tumor cells of different histogenetic origins showed that MRP is predominantly located in the plasma membrane. Immunoelectron micros copy confirmed the plasma membrane location of MRP. The MRP anti bodies provide a sensitive and specific tool for studies on MRP-mediated multidrug resistance.
منابع مشابه
Immunochemical detection of the multidrug resistance-associated protein MRP in human multidrug-resistant tumor cells by monoclonal antibodies.
We have generated rat and murine monoclonal antibodies against multidrug resistance-associated protein (MRP), a M(r) 180,000-195,000 membrane glycoprotein involved in a non-P-glycoprotein multidrug resistance of human tumor cells. The antibodies were raised against two different segments of MRP and found to be suitable for protein blot analyses, immunohistochemical and cytochemical studies, as ...
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We have generated rat and murine monoclonal antibodies against multidrug resistance-associated protein (MRP), a M,. 180,000-195,000 membrane glycoprotein involved in a non-P-glycoprotein multidrug re sistance of human tumor cells. The antibodies were raised against two different segments of MRP and found to be suitable for protein blot analyses, immunohistochemical and cytochemical studies, as ...
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